
RESEARCH SCOPE
Contemporary evolution is a common occurrence in biological invasions. For example, many common garden experiments report that non-native populations show higher competitive ability than native populations. However, our understanding of contemporary evolution is limited because most studies underestimate among-population variation (APV) within and between native and non-native ranges. More specifically, only a few studies on contemporary evolution disentangle how population histories drive APV. Furthermore, integrative frameworks of APV-focused research are lacking but could identify mechanisms of contemporary evolution, particularly with a view on state-of-the-art population genomics, ecometabolomics, and metagenomics assessing belowground biotic interactions. To address this research gap, our collaborative network performed a greenhouse experiment testing for the determinants of APV in competitive ability in a multi-omics study. Doing so, we aim to develop a more mechanistic understanding of how contemporary evolution drives competitive ability across native and non-native ranges.

METHODS
We performed a greenhouse experiment with 852 seed families from 108 native and 176 non-native populations in a competition × drought treatment combination. Field data served as proxies for population history, encompassing competitive regime (i.e., plot biomass), drought regime (i.e., aridity index), and fungal interactions in the rhizosphere (i.e., mutualist-pathogen-ratio). The samples underwent analyses across four integrative work packages (WPs) in a coherent manner.
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WP1: Phenotypic performance was assessed to examine APV in competitive ability under dry and mesic conditions.
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WP2: APV in root exudate profiles (analyzed via mass spectrometry) and APV in allelopathic effects (assessed through phytometer analyses) were analyzed.
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WP3: Various root traits were recorded and amplicon sequencing of root-colonizing fungi was conducted to investigate APV in root-fungal interactions.
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WP4: Seed families underwent genotyping using ddRADseq.

OVERALL AIMS
Principles of rapid evolution:
Investigating how population history determines APV under common garden conditions for competitive ability, drought responses, root exudate profiles, allelopathic activity, resource acquisition patterns and defence mechanisms against fungal pathogens.
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Functioning of competitive ability:
Studying correlations between the investigated APVs (i.e., across our interdisciplinary WPs) to unravel how belowground mechanisms determine competitive ability, including genome-wide association studies to identify genomic regions that drive APV in competitive ability.